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The Application of TLC Analysis in Plant Extract Detection

author:Naturalindate:2014-06-24 09:26Click: times
Thin-layer chromatography (TLC) is an important and simple technique used to rapidly separate and qualitatively analyse a few of substances. Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of stationary phase, after the sample to be analyzed or separated has been applied on the thin plate, put it into an enclosed tank and add a proper amount of solvent, which would be spread as mobile phase. Because of capillary tube, the solvent will be absorbed, move along the plate and flow across the sample; then all constituents of the sample will be driven forward by the solvent, due to diffeent physicochemical properties and absorption effect of all constituents, their movement distance may also differ from each other; upon a certain distance, all constituents will separate from each other, develop color with color developing agent.

Herb extract is a kind of products that using herbs as the raw material, through the process of extraction and separation, directional obtaining and extracting one or more effective ingredients in plants, and no changing its effective components structure at the same time in accordance with the extraction purpose. Herb extract is natural and little side effect, thus it has important value in research of medicine, food and chemical industry. It usually takes morphological identification, physical and chemical identification and spectral chromatographic analysis to identify authenticity of herb extract. And TLC is a kind of solid-liquid adsorption chromatography, it has both the advantages of column chromatography and paper chromatography. On the one hand, it is suitable for the separation of a few of samples. On the other hand, it can also be used to refine samples, just by thickening the adsorption layer when preparing thin layer plate. This method is particularly suited to the herb extract which is less volatile or changes easily under higher temperature and can't be analyzed with the gas chromatography. In recent years, TLC is widely applied to the analysis and identification of organic compounds, separation and refining, organic synthesis, structural analysis, biometrics, etc. of effective parts of plant medicine. Especially in the research and development of effective parts of plant medicine and quality control of Chinese patent medicine, it is the simplest scientific method of qualitative and quantitative analysis.

Currently, except chemical reference substance that is used as index reference of medicine identification in Chinese Pharmacopoeia TLC identification, there are chemical markers and whole chromatography of the reference used as reference, which solves the problem that a single chemical marker may not fully reflect the overall characteristics of herbs and the problem that the chemical composition which some species of plants coexist is not specifically attributed. Therefore, we use this way to qualitatively identify the plant materials and extracts. Take garlic extract as the example, by using extracts with different proportions for TLC, you can clearly see that the spot is obviously different based on the different raw materials and ratios. In the herb extract industry, there is few company which can carry out the TLC analysis of each products, however, our Naturalin company are studying how to add the TLC to our quality standards. TLC of herbal medicine identified in recent two years is as shown in Fig. 1 below.
garlic extract,TLC Analysis
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